To enhance the thrombolytic diagnosis and treatment capabilities of medical institutions in Southwest China, strengthen multi-disciplinary collaboration, and improve the prognosis of stroke patients, the "Southwest Thrombolysis Therapy Academic Conference (Sessions 2-5)" was successfully held from May to September 2025, hosted by the Chengdu High-tech Zone Medical Association. The conference focused on cutting-edge topics such as thrombolytic therapy and conducted in-depth case discussions on clinical challenges, providing a communication platform for healthcare practitioners from multiple regions in Southwest China. During the event, participants fully shared clinical practices and quality control experience, effectively promoting the coordinated development of stroke prevention and treatment in the region. The conference further strengthened the multi-disciplinary collaboration mechanism and laid a solid foundation for improving the overall quality of regional stroke diagnosis and treatment.